Progress in autoantibodies to platelet and idiopathic thrombocytopenic purpura

The onset of idiopathic thrombocytopenic purpura (ITP) is related to excessive destruction of platelet resulting from antiplatelet autoantibody. The immunity with ITP is imbalance, so the hypofunction of suppressor T cell (Ts) can't restrain B lymphocytes producing antibody, as a result, autoant ibody is produced, the clearance of platelet in blood circulation is accelerated. The different antibodies have different functions on ITP. The measuring of platelet antibody has its significance in diagnosis, therapeutic evaluat ion and expectation of prognosis.     

Effect of antifibrotic herb serum on rat hepatic stellate cell proliferation and collagen synthesis

AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[³H]H]proline and [[³H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P＜0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P＜0.05). Portal serum decreasea collagen synthesis (P＜0.05), but circumferential serum had no effect (P＞0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action.     

细胞骨架解聚药物对小麦与叶锈菌互作诱发的细胞过敏性反应的影响

 小麦(Triticum aestivum)品种洛夫林10和叶锈菌小种366组成不亲和组合,小麦叶片发生过敏性坏死反应(HR)是小麦抵抗叶锈菌侵染的重要因素。在接种前给小麦叶片分别预注射微管解聚药物磺草硝(oryzalin)和微丝解聚药物细胞松弛素D (cytochalasin D,CD),结果表明2种药物注射使得寄主因叶锈菌侵染诱导的细胞过敏性坏死数目明显减少,并且注射药物的浓度越大,寄主细胞发生HR的数量越少。说明肌动蛋白和微管蛋白的聚合状态是诱发小麦叶片发生HR防卫反应所必需的,细胞骨架在小麦抵抗叶锈菌侵染过程中可能起着重要作用。     

离体条件下水稻种衣剂对串珠镰刀菌生长及形态毒理影响

 检测了黑龙江水稻主产区4个当地主栽品种的种子内部镰刀菌寄藏情况,测定了20%克福甲和20%克多甲种衣剂对种子带菌消毒处理效果及对水稻串珠镰刀菌(Fusarium moniliforme)的抑菌作用和联合毒力,并借助扫描电镜观察了上述2种混配种衣剂对串珠镰刀菌的形态毒理影响。结果表明,种子内部镰刀菌的分离频率高达56.7%~96.0%,其中串珠镰刀菌的分离频率为32.6%~48.2%,2种种衣剂对带菌种子具有显著的消毒处理效果。20%克福甲和20%克多甲种衣剂对镰刀菌F.moniliforme的毒力指数分别为457.11和802.04,增效倍数分别为6.53和0.13。20%克多甲种衣剂(多菌灵:甲基立枯磷为5:5,W/W)对串珠镰刀菌的抑菌作用优于20%克福甲种衣剂(福美双:甲基立枯磷为8:6,W/W),增效作用低于20%克福甲种衣剂。电镜观察表明,种衣剂低浓度至高浓度处理下均可引起串珠镰刀菌菌丝不同程度的异常生长,表现为主菌丝局部膨大或形成菌丝束,菌丝顶端异常膨大、缢缩或形成穗状和花絮状分枝。     

植物杀虫剂苦皮藤素对柑橘潜叶蛾控制效果的研究

用植物性杀虫剂苦皮藤素Ⅳ(KPT乳油)有效成分30、15mg/kg防治柑橘潜叶蛾,采用离体叶片浸叶法处理5s,3d后,幼虫校正虫口减退率分别为89.63%、63.02%;枝梢浸液法处理5s,5d后,潜道伸长度校正抑制率分别为76.12%和41.51%;10d后,对秋梢的相对保叶效果分别为69.34%和52.29%。结果表明,采用KPT乳油有效成分30mg/kg对柑橘潜叶蛾有较好的控制作用,对柑橘秋梢有良好的保护效果。     

等值线制图工具 Surfer在病虫测报上的应用研究

Surfer系统是美国金软公司开发的专门制作等值线的工具。1997年以来,笔者对其运用于稻飞虱等农作物病虫害的监测方面作了探讨和开发。阐述了病虫分布图的绘制和遮盖图编制的过程和方法。     

姬松茸与双孢蘑菇不同菌株的RAPD扩增研究

以4个姬松茸菌株和2个双孢蘑菇菌株为材料，用20个随机引物对它们进行RAPD扩增，通过对供试菌株遗传相似系数的估算和系统聚类分析，构建姬松茸和双孢蘑菇遗传相关树状聚类图谱，结果表明，进行RAPD扩增的20个随机引物中，有12个能产生三种带型，当遗传相似系数升至0.8713时，这6个菌株被分为3类，第一类为菌株18和26，第二类为菌株13和12，第三类为菌株33和45。     

营养和环境条件对杏鲍菇菌丝生长的影响

研究了杏鲍菇菌丝生长的营养需求和环境条件，结果表明，适宜杏鲍菇菌丝生长的最佳碳源是葡萄糖、最佳氮源是黄豆粉，最佳碳氮比是60：1，最佳无机盐是硫酸钙。杏鲍菇菌丝生长的适宜温度为25℃，培养料最适含量为75%，最适pH为5.4。     

越冬橘园蜘蛛群落多样性研究

通过系统调查,湖南省邵阳市越冬橘园蜘蛛群落共有12科22属28种,其中斑管巢蛛(Clubionareichlini)和美丽蚁蛛(Myrmarachaefarmicaria)为优势种。异质性大的越冬橘园蜘蛛群落的多样性指数、丰富度和个体总数均大于异质性小的越冬橘园,越冬橘园蜘蛛群落的多样性指数大小主要由群落物种丰富度和个体总数决定,与均匀度大小无关。